The fresh SNP indicators (lm1712, np2623, and you will np2691) nearest to the peaks from around three QTLs that turned out steady grizzly for ? thirteen C round the many years significantly less than drought be concerned have been putatively discovered in order to Chromosome 8 (Chr8): 12659110, Chr15: 36996403, and you may Chr16: 4666838 centered on Fruit Genome v1.0. Our very own research regarding results from KASP (Fig. 3a) and you may RADseq genotyping of the identical progenies revealed extremely consistent costs for these seedlings, i.e., , , and you can % having indicators lm1712, np2623, and you can np2691, correspondingly (Table 6).
Validation of QTLs for ? 13 C under drought-stressed conditions, based on genotyping using KASP assay-based SNP markers. a genotype plot on 3 positions (lm1712, np2623, and np2691) for KASP platform.